In this work, we describe a single piggyBac transposon system containing both a tet-activator Dryer Timer Knob and a doxycycline-inducible expression cassette.We demonstrate that a gene product can be conditionally expressed from the integrated transposon and a second gene can be simultaneously targeted by a short hairpin RNA contained within the t
Abstract The cell cycle is tightly controlled to ensure that replication origins fire only once per cycle and that consecutive S-phases are separated by mitosis.When controls fail, DNA over-replication ensues: individual origins fire more than once per S-phase (re-replication) or consecutive S-phases occur without intervening mitoses (endoreduplica
As the time passes from the confiscation of the property, and on the basis of the regulations listed in the Law on property Restitution and Compensation, the possibility to achieve the basic purpose of this law has been diminished, due to legal and factual changes in relation to the property, and change of generations.Additional reasons for the les